fmlp induced chemokine gene expression in hmc-1 cells
| PAG Title | fmlp induced chemokine gene expression in hmc-1 cells |
| PAG ID | WAG000831 |
| Type | P |
| Source Link |  BioCarta |
| Publication Reference | NA |
| PAG Description | Neutrophils respond to bacterial infection by releasing reactive oxygen species that kill bacteria and by expressing chemokines that attract other immune cells to the site of infection. The multisubunit enzyme DPH oxidase expressed by neutrophils produces reactive oxygen species rapidly released in what is known as the respiratory burst. Activity of the DPH oxidase is induced by fMLP receptor ligands, formylated peptides from bacteria. The fMLP receptor is a G-protein coupled receptor, FPR-1, that activates Map kise pathways and phospholipase C. Phospholipase C activation releases IP3 and calcium, activating protein kise C and also activating the transcription factor NFAT, which contributes to activation of chemokine genes. One of the components of the DPH oxidase is p47phox. PKC activation phosphorylates p47phox to activate DPH oxidase activity. Activation of Map kise cascades leads to Erk1/Erk2 dependent p47phox phosphorylation as well as activation of the Elk-1 transcription factor and chemokine gene expression. Inhibition of p38 did not affect p47phox phosphorylation, indicating that p38 is not involved in Erk1/2 activation of the DPH oxidase. Inhibition of p38 did inhibit DPH oxidase though, indicating that other pathways contribute to activation of this enzyme. The pathways involved in neutrophils activation are important to understand inte immune responses to bacterial infection. |
| Species | Homo sapiens |
| nCoCo Score | 2,403 |
| Base PAG ID | WAG000831 |
| Human Phenotyte Annotation | |
| Curator | PAGER curation team |
| Curator Contact | PAGER-contact@googlegroups.com |
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